Bd tellurite agar hoyle is a modification of the medium developed by neill for the. It is primarily used in processing of food, cosmetics and environmental samples rather than clinical samples. Cystine tellurite blood agar will appear hemolyzed due to the high tellurite content. Potassium tellurite for microbiology catalog codes. The chemical composition of nutrient agar sciencing. Mechanism behind cystinetellurite blood agar essay sample. Potassium tellurite is used together with agar as part of a selective medium for growth of some bacteria clauberg medium. Oxoid potassium tellurite thermo fisher scientific. Cefixime potassium tellurite sorbitolmacconkey agar.
The medium appears cream to yellow colored, and takes the form of a freefloating powder. While evolving molecular diagnostic methods are being heralded for the role they will play in improving our ability to cultivate and identify bacteria, fungi, and viruses, the reality is that those new methods are still beyond the technical and financial reach of most clinical laboratories. The number of electrons in each of potassiums shells is 2, 8, 8, 1 and its electron configuration is ar 4s 1. Although these solutions can be mixed to be added to the baird parker agar base forming the commonly known egg yolk tellurite sterile emulsion ref. Tellurite blood agar is a selective medium used for isolation and cultivation of corynebacterium species. As a result of this investigation, the routine use of cystineserum tellurite medium is recommended.
This products resides on a fisher scientific gsa or va contract. Inoculate a tube of middlebrook 7h9 broth w summary and explanation in 1969, kilburn et al. Agar consists of a mixture of sugar polymers polysaccharides, where the basic sugar is galactose. Cm0507 macconkey agar without salt cm0523 giolitticantoni broth. Thermo scientific oxoid potassium tellurite 10 x 2ml. Potassium tellurite is also a selective agent which inhibits a variety of grampositive. Cefixime potassium tellurite sorbitolmacconkey agar how.
In addition to possessing those prime virtues, simplicity and low cost, the medium is selective to a high degree due to the presence of potassium tellurite. Hardy diagnostics macconkey agar with sorbitol, cefixime, and tellurite ctsmac is used for the selective and differential isolation of enterohemorrhagic escherichia coli o157. Potassium was discovered and first isolated by sir humphrey davy in 1807. Why does potassium permanganate diffuse faster in agar gel.
A mineral with the formula teo 2 is called tellurite. Pdf role of potassium tellurite and brain heart infusion. The influence of potassium tellurite pt and brain heart infusion agar difco, two components of modified listeria selective agar medium lsamm, on the hemolytic phenotype of listeria spp. Corynebacteria and some other species reduce teo 2. Get a printable copy pdf file of the complete article 921k, or click on a page image below to browse page by page. Nile chemicals,manufacturers, exporters, stains, ph indicators, chemicals, drugs, aluminium chloride, aluminum chloride, dyes, basic dyes, metal salts, phase.
Links to pubmed are also available for selected references. If you are viewing this page as a nonregistered user, the prices displayed is list price. One was done in a petri dish containing agar gel along with methylene blue. Throat or nasal swab is directly inoculated and streaked on this agar medium. They are typically colorless or white salts, which in some ways are comparable to sulfite. This is a sterile emulsion of egg yolk in saline containing potassium tellurite and is generally used as a selective differential agent in baird parker medium. Lithium chloride and potassium tellurite acts as inhibitor agent for contaminating microflora. Ingredients concentration potassium tellurite concentrate 1. Potassium tellurite solution for use with modified giolitti and cantoni broth iso lab219 for the detection. Mannitol salt agar and salt milk agar used to recover s. Agar is a gelatinous mixture that is extracted from seaweed. They also found modified tinsdale medium as satisfactory as cystinetellurite blood agar for the primary isolation of c.
Macconkey agar with sorbitol, cefixime and tellurite ctsmac. Baird parker agar was developed by baird parker in 1962 and is a modification of telluriteglycine formulation of zebovitz et al. Baird parker agar principle, preparation and uses learn. Serum tellurite agar is a selective and differential medium used for isolation of members of the genus corynebacterium, particularly in the laboratory diagnosis of diphtheria. Potassium tellurite solution for use with modified giolitti and cantoni broth iso lab219 for the detection and enumeration of coagulasepositive staphylococci. Bd tellurite agar hoyle intended use bd tellurite agar hoyle is a partially selective and differential medium for the isolation of corynebacterium diphtheriae from clinical specimens. Meat extract and peptone supply nitrogen and growth factors. Most clinical microbiology laboratories still rely upon cu.
Mechanism behind cystinetellurite blood agar essay example for free newyorkessays database with more than 65000 college essays for studying. As a result of this investigation, the routine use of cystineserumtellurite medium is recommended. Potassium tellurite 1% final concentration after addition of 8. Search results for potassium tellurite at sigmaaldrich. Molecular weight of potassium tellurite convert units. Composition to achieve 1% solution dilute the contents in 8. Corynebacteria and some other species reduce teo 32 to elemental te, which stains the bacteria black. Tinsdales original agar medium 1 containing serum, tellurite, cystine and formolised blood was formulated to differentiate between corynebacterium diphtheriae and the diphtheroids found in the upper respiratory tract. It is a moderately selective medium for the isolation and differentiation of coagulase positive staphylococci especially staphylococcus aureus. Use of a potassium tellurite medium in the detection of. The number of electrons in each of potassium s shells is 2, 8, 8, 1 and its electron configuration is ar 4s 1. Organism names see more detailsdysentery dysentery subject category. The complete medium is selective for staphylococcus aureus as the potassium tellurite inhibits most coliform organisms and is also reduced by staphylococcus aureus to tellurite giving typical black colonies on the baird parker medium. Potassium tellurite k 2 teo 3 is used together with agar as part of a selective medium for growth of some bacteria clauberg medium.
A sterile egg yolk emulsion containing potassium tellurite for use in bairdparker medium base iso lab285 or bairdparker medium base lab085. Bairdparker agar is a selective medium for the enumeration of staphylococcus aureus in foods and this was first reported by bairdparker. Full text full text is available as a scanned copy of the original print version. Pathogenic strains of corynebacteria can infect plants, animals, or humans. The chapter discusses bairdparker agar composition, which consist of tryptone, beef extract, yeast extract, lithium chloride, sodium pyruvate, glycine, potassium tellurite, sulphamezathine, egg yolk emulsion, agar, and distilled or deionized water. The evaluation of a differential medium cystineserumtellurite agar for isolation and identification ofcorynebacterium diphtheriae is reported. Convert grams potassium tellurite to moles or moles potassium tellurite to grams. Principles and explanation of the procedure microbiological method.
In this paper a study of the relations of cystine and tellurite in this medium were studied. The relative tolerance to potassium tellurite of various gramnegative bacilli, mostly of the colisalmonelladysenteryproteus fraternity, was tested in broth and on agar. Diseases, disorders, and symptoms see more detailsproteus fraternity, was tested in broth and on agar. Glucosesucrosepotassium telluritebacitracin agar, an. Selectivity is attained with potassium tellurite and lithium chloride. According to my observations, the potassium in the small petri dish reached equilibrium faster than its counter part in the waterfilled cylinder. Ctsmac cefixime potassium tellurite sorbitolmacconkey agar. Description oxoid tinsdale base is used with oxoid tinsdale supplement sr0065 for the primary isolation and identification of corynebacterium diptheriae. Tellurite blood agar is a selective medium used for isolation and cultivation of corynebacterium species 1, 2. The cultures were classified as very sensitive inhibited by 0. The tellurite additive is toxic to egg yolkclearing strains other than s. Streak the tinsdale agar plate to obtain wellisolated colonies.
The inhibitory action of potassium tellurite on coliform. Potassium is the seventh most abundant element on earth. Potassium tellurite for microbiology 90% 25gmbtlk2teo3, potassium tellurate iv, telluric acid dipotassium salt. A formulation of the new medium containing 5% glucose 5% sucrose, 0. It is widely recommended by national and international bodies. It is selective due to the presence of inhibitor and differential by means of ability of organism to reduce potassium tellurite. Components like casein enzymic hydrolysate, meat extract and yeast extract provides nitrogen, carbon, sulphur and vitamins. It is cefixime potassium tellurite sorbitolmacconkey agar. It was found that excessive quantities of cystine, in the presence even of minute amounts of tellurite, are definitely. In a previous paper mcguigan and frobisher this bulletin, 1936, v. Hoyles agar is a selective medium that uses tellurite to differentially select corynebacterium diphtheriae from other upper respiratory tract flora.
The tinsdales agar base medium without serum and tellurite is stable indefinitely if stored in closed screwcapped tubes or bottles but after the addition of supplement serum and tellurite, the medium is stable only for 23 days if stored in the refrigerator. Recommended for the selective isolation of staphylococci and corynebacteria. The other one was in a tall cylinder filled with water. A tellurite compound, for example sodium tellurite, is a compound that contains this ion. Peptone agar base m734 dextrose proteose peptone hiveg agar base mv734 cystine tellurite agar base. Potassium tellurite for microbiology 123333664 100. Potassium tellurite acts as a selective agent and has inhibitory activity against most grampositive and gramnegative bacteria except corynebacterium species.
Corynebacterium diphtheriae on tellurite agar medical. With these findings, they recommended the use of modified tinsdale medium for routine isolation of c. The evaluation of a differential medium cystineserum tellurite agar for isolation and identification ofcorynebacterium diphtheriae is reported. Baird parker agar medium is formulated on the principle that staphylococci are able to reduce tellurite to tellurium and to detect lecithinase from egg lecithin. Bd tellurite agar hoyle is a modification of the medium developed by neill for the isolation of. Potassium tellurite k2teo3is used together with agar as part of a selective medium for growth of some bacteria. If material is being cultured directly from a swab, roll the swab over a small area of the agar surface and streak the plate for isolation. The addition of cystine greatly enhances the growth of c. Learn vocabulary, terms, and more with flashcards, games, and other study tools.